Isolation and Characterization of Actinomycetes from Plant Rhizosphere in Gorontalo Karst Area
DOI:
https://doi.org/10.61579/mikhayla.v2i1.286Keywords:
Actinomycetes, Karst, RhizosphereAbstract
Karst areas are landscapes composed of carbonate rocks and are rich in calcium and magnesium elements, making some macronutrients unable to be absorbed by plants directly. One of the soil microorganisms associated with plants in karst areas is Actinomycetes. Some species of Actinomycetes are known to produce growth hormones such as Indole Acetic Acid and dissolve phosphate bound in the soil. Actinomycetes also play an important role in soil ecosystems, especially in areas with extreme environmental conditions such as karst areas. Karst areas are known for their unique biodiversity and the potential of microorganisms that have not been fully explored. Gorontalo karst area is a potential location for the exploration of these microorganisms. This study aims to isolate and characterise Actinomycetes found in the rhizosphere of plants in Gorontalo karst area. Sampling was carried out on 9 plant species found in two locations namely Tanjung Kramat, Hulonthalangi District and Karst Mountains. Macroscopic characterisation results showed that the Actinomycetes isolates obtained had 2 forms, namely Irregular (5 isolates) and Circular (4 isolates) with mycelium colour dominated by greyish black - brown, orange-orange, yellow-orange, grey-yellow, and white - yellow. Microscopic characterisation showed the results of all isolates obtained were blue or purple with thin filamentous cells indicating the isolates were Actinomycetes.
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Copyright (c) 2025 Citra Leonita Matalauni, Yuliana Retnowati, Abubakar Sidik Katili, Ani M. Hasan, Novri Youla Kandowangko
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