Isolation and Characterisation of Actinomycetes in the Rhizosfer Pandanus ammaryllifolius Plants in Gorontalo

Annisa Dwiyani; Yuliana Retnowati; Abubakar Sidik; Novri Y. Kandowangko; Wirnangsi Din Uno

doi:10.61579/mikhayla.v2i1.285

Authors

Annisa Dwiyani Universitas Negeri Gorontalo, Gorontalo, Indonesia
Yuliana Retnowati Universitas Negeri Gorontalo, Gorontalo, Indonesia
Abubakar Sidik Universitas Negeri Gorontalo, Gorontalo, Indonesia
Novri Y. Kandowangko Universitas Negeri Gorontalo, Gorontalo, Indonesia
Wirnangsi Din Uno Universitas Negeri Gorontalo, Gorontalo, Indonesia

DOI:

https://doi.org/10.61579/mikhayla.v2i1.285

Keywords:

Actinomycetes, Pandanus ammaryllifolius, Rhizosphere

Abstract

Actinomycetes are a group of gram-positive bacteria known to have high bioactivity, including the potential to produce antibiotics and other bioactive compounds. These microorganisms have cosmopolitan nature and adaptability in various soil habitats indicating that they can be found and adapt well in various types of soil. This study aims to isolate and characterise Actinomycetes bacteria from the rhizosphere of Pandanus ammaryllifolius plants in Gorontalo. The rhizosphere, which is the soil segment around plant roots, is rich in microorganisms that play an important role in nutrient cycling and ecosystem health. Sampling was conducted in two locations, namely the plantations of Pahu Village, Asparaga District, and Bone Bolango, Tilongkabila District. Isolation results using the spread plate method on Starch Casein Agar (SCA) media, showed the presence of Actinomycetes obtained 9 isolates of Actinomycetes. The highest number of isolates was found in the Bone Bolango plantation location, which was 6 isolates. Meanwhile, 3 other isolates were found in Pahu Village. Environmental conditions at the Pahu Village site had a pH of 4.4 and humidity of 5.7%, while the soil at the Bone Bolango plantation site had a pH of 7.9 and humidity of 6.8%. Morphological characterisation results showed variations in colony shape, size and colour. Actinomycetes isolates obtained were small with irregular and circular colony shapes. The colour of the colony substrate mycelium was dominated by yellow, orange, white, blackish yellow, and greyish white. The Morphological characteristics found in this isolate indicated three potential groups of Actinomycetes: Streptomyces, Micromonospora, and Micrococcus. All isolates were found to be Gram positive.

References

Alwi M, Suharjono, Ardyati, Subandi. (2020). Eksplorasi Actinomycetes Sebagai Kandidat Antibakteri Patogen Yang Resisten Dari Rhizosfer Tumbuhan Leda (Eucalyptus Deglupta Blume.) Di Taman Nasional Lore Lindu, Indonesia. Biocelebes. 14(3): 253-267, https://doi.org/10.22487/bioceb.v14i3.15310

Anggraini W. (2015). Isolasi Actinomycetes dengan menggunakan metode skrining sebagai penghasil enzim kitinase. Jurnal Ilmiah Pendidikan Fisika Al-Biruni. 4(1):85-95. DOI: 10.24042/jpifalbiruni.v4i1.83

Arum W.G., (2017). Pemetaan Tahanan Pentanahan Berdasarkan Jenis Tanah Di Provinsi Gorontalo. Skripsi. Universitas Negeri Gorontalo: Teknil Elektro

Astuty E. 2017. Isolasi dan Karakterisasi Morfologi Aktinomisetes Indegenus Asal Tanah Gambut. Jurnal Ilmu Alam dan Lingkungan. 8(16):7-15.

Badan Standardisasi Nasional. (2014). Agens Pengendali Hayati (Aph)-Bagian 3 : Trichoderma spp. SNI 8027.3:2014

Bonanomi, G., De Filippis, F., Cesarano, G., La Storia, A., Ercolini, D., & Scala, F. (2016). Organic farming induces changes in soil microbiota that affect agro-ecosystem functions. Soil Biology and Biochemistry, 103(4): 327-336

Buedenbender, L., Carroll, A. R., Ekins, M., & Kurtböke, D. I. (2017). Taxonomic and metabolite diversity of Actinomycetes associated with three Australian ascidians. Diversity, 9(4), https://doi.org/10.3390/d9040053

Dhanasekaran D, Jiang Y. (2016). Actinobacteria: Basic and Biotenichal Applications. Ave4eva Movimix Records.

DOI: 10.1016/j.soilbio.2016.09.005

Gopalakrishnan, S., Srinivas, V., & Prasanna, S. L. (2020). Streptomyces. In beneficial microbes in agro-ecology (pp. 55-71). Academic Press. DOI: 10.1016/B978-0-12-823414-3.00005-8

Katili A.S., And Y. Retnowati. (2017). Isolation Of Actinomycetes From Mangrove Ecosystem In Torosiaje, Gorontalo, Indonesia. Biodiversitas. 18(2): 826- 833, https://doi.org/10.13057/biodiv/d180259

Lestari S, Mukarlina, Kurniatuhadi R. (2019). Identifikasi dan Deteksi Aktivitas Daya Hambat Bakteri Actinomycetes yang diisolasi dari Tanah Gambut di Desa Tajok Kayong Kalimantan Barat. J Protobiont.. 8(1):13–19. DOI: 10.26418/protobiont.v8i1.30843

Iwin HT, Yin ZZ, Mya TM. (2020). Isolation and Antimicrobial Activity of Actinomycetes From Chaung Tha Area And Biochemical Characterization of Selected Streptomyces (TR-2). Journal Myanmar Acad. Arts Sci. 18 (4B): 145-158.

Murray PR, Rosenthal KS, Pfaller MA. (2016). Medical Microbiology. 8th ed.. Kanada: Elseiver. 106–118 p

Nurjasmi, R., dan Suryani. (2017). Uji Antagonistik Actinomycetes Asal Limbah kulit Bawang Merah Terhadap Patogen Tanaman. Jurnal ilmiah Respati Pertanian. 11(2): 718-722, https://doi.org/10.52643/jir.v8i2.76

Prayudyaningsih, A., Nursyamsi dan S. Ramadana. (2015). Mikroorganisme Tanah Bermanfaat bagi Rizosfer Tanaman Umbi dibawah Tegakan Hutan Rakyat Sulawesi Selatan. Balai Penelitian Hutan Makassar. 1(4): 954-959.

Raza, W., Ling, N., Yang, L., Huang, Q., & Shen, Q. (2016). Response of tomato wilt pathogen Ralstonia solanacearum to the volatile organic compounds produced by a biocontrol strain Bacillus amyloliquefaciens SQR-9. Scientific reports. 6(1): 24856. DOI: 10.1038/srep24856

Rozirwan, Muda, H.I., Ulqodry, T. Z. (2020). Antibacterial Potential of Actinomycetes Isolated from Mangrove Sediment in Tanjung Api-Api South Sumatra. Biodiversitas. 21(12): 5723-5728, https://doi.org/10.13057/biodiv/d211232

Saputri, K. E., Idiawati, N., dan Sofiana, M. S. J. (2021). Isolasi dan Karakteristik Bakteri Penambat Nitrogen dari Rizosfer Mangrove di Kuala Singkawang. Jurnal Laut Khatulistiwa. 4(2): 17–21, http://dx.doi.org/10.26418/lkuntan.v4i2.45316

Setyawati, T.R., R. Kurniatuhadi dan A.H. Yanti. (2021). Karakter Morfologi Koloni Streptomyces spp. yang Diisolasi dari Substrat Habitat Cacing Nipah (Namalycastis rhodochorde) pada Medium Berbeda. Seminar Nasional Penerapan Ilmu Pengetahuan dan Teknologi 2021. Pontianak, Indonesia: Universitas Tanjungpura.

Sofariyanti AE, Sasongkowati R, Anggraini AD. (2019). Aktivitas antibakteri aktinomisetes di Hutan Mangrove Wonorejo Surabaya yang antagonis terhadap bakteri Staphylococcus aureus. Anal Kesehat Sains. 8(2):738–748.

Tan, G. Y., Peng, Y., Lu, C., Bai, L., & Zhong, J. J. (2015). Engineering validamycin production by tandem deletion of γ-butyrolactone receptor genes in Streptomyces hygroscopicus 5008. Metabolic Engineering, 28, 74–81, https://doi.org/10.1016/j.ymben.2014.12.003

Tyas SP. (2020). Optimasi Pertumbuhan Isolat Actinomycetes (Isolat TE 235) dan Uji Aktivitas terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Skripsi. Program Studi Farmasi, Fakultas Ilmu Kesehatan. Universitas Muhammadiyah. Magelang.

Yunus, F., Lambui, O., & Suwastika, I. N. (2017). Kelimpahan mikroorganisme tanah pada sistem perkebunan kakao (Theobroma cacao L.) semi intensif dan non intensif. Natural Science: Journal of Science and Technology, 6(3), https://doi.org/10.22487/25411969.2017.v6.i3.9192