Phosphate Soluting Actinomycetes in the Rhizosfer of Corn (Zea mays) in Gorontalo

Andre Putra Lamuka; Yuliana Retnowati; Abubakar Sidik Katili; Novri Youla Kandowangko; Wirnangsih Din Uno

doi:10.61579/mikhayla.v2i1.282

Authors

Andre Putra Lamuka Department of Biology, Gorontalo State University, Gorontalo, Indonesia
Yuliana Retnowati Department of Biology, Gorontalo State University, Gorontalo, Indonesia
Abubakar Sidik Katili Department of Biology, Gorontalo State University, Gorontalo, Indonesia
Novri Youla Kandowangko Department of Biology, Gorontalo State University, Gorontalo, Indonesia
Wirnangsih Din Uno Department of Biology, Gorontalo State University, Gorontalo, Indonesia

DOI:

https://doi.org/10.61579/mikhayla.v2i1.282

Keywords:

Actinomycetes, Phosphate Solubiliser, Rhizosphere, Corn (Zea mays)

Abstract

Actinomycetes are Gram-positive bacteria with morphological characteristics in the form of unicellular filaments that have the potential to dissolve bound phosphate into a form available to plants. This study aims to isolate and identify phosphate-solubilizing Actinomycetes from the rhizosphere of corn (Zea mays) in Gorontalo, Indonesia. Rhizosphere soil samples were taken at a depth of 15-30 cm from three corn farming locations in Mohiyolo Village, Asparaga District. Parameters analyzed included environmental characteristics, isolate morphology, phosphate solubility index, and soluble phosphate concentration. Identification was based on the morphology of aerial mycelium and substrate mycelium, as well as physiological tests of phosphate solubilization ability. Phosphate solubility index was calculated from the ratio of halo zone diameter to colony on Pikovskaya agar medium, while soluble phosphate concentration was measured using spectrophotometry. The results showed that RFZm-Pg isolate had the highest solubility index of 2.98 mm, while RFZm-Pw isolate was 1.54 mm. The highest dissolved phosphate concentration in liquid media was 0,8048 ppm (RFZm-Pg) and 0,4373 ppm (RFZm-Pw). The phosphate-solubilizing Actinomycetes isolates obtained have potential as biofertilizers, so further testing is needed.

References

Astuty, E. (2017) ‘Isolasidan Karakterisasi Morfologi Aktinomiset Indigenus Asal Tanah Gambut’, Jurnal Ilmu Alam dan Lingkungan, 8(2), pp. 7–15. Available https://doi.org/10.20956/jal.v8i16.2980.

Dewi, S.C.O. et al. (2024) ‘Optimalisasi Dan Uji Efektivitas Actinomycetes Pada Brassica Chinensis Di Bawah Cekaman Kekeringan Dan Ph Masam Ultisol’, Jurnal Tanah dan Sumberdaya Lahan, https://doi.org/10.21776/ub.jtsl.2024.011.1.21.

Edy, E. and Ibrahim, B. (2022) ‘Efisiensi Penggunaan Pupuk Fosfor Pada Tanaman Jagung Dengan Aplikasi Ekstrak Pelarut Fosfat’, AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 6(1), pp. 90–98. https://doi.org/10.33096/agrotek.v6i1.179.

Hartanto, P., Zulkifli, L. and Sedijani, P. (2023) ‘Jurnal Biologi Tropis Isolation and Identification of Phosphate Solubilizing Bacteria from The Rhizosphere of Dry Land Lamtoro Plants (Leucaena leucocephala) in North and South Lombok Regions’.

Herms, C.H. et al. (2022) ‘Back to our roots: exploring the role of root morphology as a mediator of beneficial plant–microbe interactions’, Environmental Microbiology, 24(8), pp. 3264–3272. https://doi.org/10.1111/14622920.15926.

Huang, X. et al. (2014) ‘Rhizosphere interactions: root exudates, microbes, and microbial communities 1’, 275 (February), pp. 267–275.

Kanti, A. (2005) ‘Cellulolytic Actinomycetes isolated from soil in Bukit Duabelas National Park, Jambi’, Biodiversitas Journal of Biological Diversity, 6(2), https://doi.org/10.13057/biodiv/d060203.

Khan, M.S., Zaidi, A. and Musarrat, J. (2014) ‘Phosphate solubilizing microorganisms: Principles and application of microphos technology’, Phosphate Solubilizing Microorganisms: Principles and Application of Microphos Technology, https://doi.org/10.1007/978-3-319-08216-5.

Larasati, E.D. et al. (2018) ‘Isolasi dan Identifikasi Bakteri Pelarut Fosfat dari Tanah Gambut’, Bioma : Berkala Ilmiah Biologi, 20(1), p. 1. https://doi.org/10.14710/bioma.20.1.1-8.

Lovitna, G., Nuraini, Y. and Istiqomah, N. (2021) ‘Pengaruh Aplikasi Bakteri Pelarut Fosfat Dan Pupuk Anorganik Fosfat Terhadap Populasi Bakteri Pelarut Fosfat, P-Tersedia, Dan Hasil Tanaman Jagung Pada Alfisol’, Jurnal Tanah dan Sumberdaya Lahan, 8(2), pp. https://doi.org/10.21776/ub.jtsl.2021.008.2.15.

Mardyansah, D. and Trimulyono, G. (2021) ‘Isolasi , Karakterisasi , dan Uji Potensi Bakteri Pelarut Fosfat dari Rhizosfer Tanaman Jati dan Sengon di Pegunungan Kapur , Daerah Selatan Kabupaten Tulungagung Isolation , Characterization , and The Potential Test of Phosphate Solubilizing Bacteria fro’, 10, pp. 188–198.

Nasamsir, N., Nengsih, Y. and Purba, H.P. (2022) ‘Kandungan Pospor-tersedia Pada Berbagai Kondisi Lahan Yang Berbeda danProduktivitasKelapa Sawit (Elaeis guineensis Jacq.)Afdeling IV Rimsa PTPN VI Persero Rimbo Bujang Kabupaten Tebo Jambi’, Jurnal Media Pertanian, 7(1), p. 11. https://doi.org/10.33087/jagro.v7i1.142

Nash, D.M. et al. (2014) ‘Using organic phosphorus to sustain pasture productivity: A perspective’, Geoderma, 221–222, pp. 11–19. https://doi.org/10.1016/j.geoderma.2013.12.004.

Niswati, A., Yusnaini, S. and Syamsul, A. (2008) ‘Populasi Mikroba Pelaru Fosfat dan P-tersedia pada Rizorfir beberapa Umur dan Jarak dari Pusat Perakaran Jagung (Zea mays L.)’, Jurnal Tanah Tropika, 13(2), pp. 123–130.

Nurkanto, A. (2007) ‘Identification of soil actinomycetes in Bukit Bangkirai fire forest East Kalimantan and its potention as cellulolitic and phosphate solubilizing’, Biodiversitas Journal of Biological Diversity, 8(4), pp. 314–319. https://doi.org/10.13057/biodiv/d080414.

Pan, F. et al. (2024) ‘Vegetation recovery reshapes the composition and enhances the network connectivity of phoD-harboring microorganisms to promote P availability in a karst ecosystem’, Science of the Total Environment, 918(January), https://doi.org/10.1016/j.scitotenv.2024.170561.

Paul, D. and Sinha, S.N. (2017) ‘Isolation and characterization of phosphate solubilizing bacterium Pseudomonas aeruginosa KUPSB12 with antibacterial potential from river Ganga, India’, Annals of Agrarian Science, pp. 130–136. https://doi.org/10.1016/j.aasci.2016.10.001

Purba, M.A., Fauzi and Kemala, S. (2015) ‘Pengaruh Pemberian Fosfat Alam dan Bahan Organik pada Tanah Sulfat Masam Potensial Terhadap P-Tersedia Tanah dan Produksi Padi (Oryza sativa L.)’, Jurnal Online Agroekoteaknologi, 3(3), pp. 938–948.

Puspitasari, H.M., Yunus, A. and Harjoko, D. (2018) ‘Dosis Pupuk Fosfat Terhadap Pertumbuhan dan Hasil Beberapa Jagung Hibrida’, Agrosains: Jurnal Penelitian Agronomi, 20(2), p. 34. https://doi.org/10.20961/agsjpa.v20i2.22058.

Rangseekaew, P. and Pathom-Aree, W. (2019) ‘Cave actinobacteria as producers of bioactive metabolites’, Frontiers in Microbiology, 10(MAR). Available at: https://doi.org/10.3389/fmicb.2019.00387.

Ratih, R., Saida, S. and Nontji, M. (2022) ‘Pertumbuhan Rhizobakteri Asal Rhizosfer Tanaman Jagung (Zea Mays L.) Pada Berbagai Media Organik Cair’, AGrotekMAS Jurnal Indonesia: Jurnal Ilmu Peranian, 2(2), pp. 1–10. https://doi.org/10.33096/agrotekmas.v2i2.185.

Retnowati, Y., Kandowangko, N.Y. and Katili, A.S. (2024) ‘Diversity of actinomycetes on plant rhizosphere of karst ecosystem of Gorontalo , Indonesia’, 25(3), https://doi.org/10.13057/biodiv/d250301.

Selim, M.S.M., Abdelhamid, S.A. and Mohamed, S.S. (2021) ‘Secondary metabolites and biodiversity of actinomycetes’, Journal of Genetic Engineering and Biotechnology, 19(1). https://doi.org/10.1186/s43141-021-00156-9.

Shirinbayan, S., Khosravi, H. and Malakouti, M.J. (2019) ‘Alleviation of drought stress in maize (Zea mays) by inoculation with Azotobacter strains isolated from semi-arid regions’, Applied Soil Ecology, 133(September), pp. 138–145. https://doi.org/10.1016/j.apsoil.2018.09.015.

Solihin, M.A. and Fitriatin, B.N. (2017) ‘Sebaran Mikroba Tanah pada Berbagai Jenis Penggunaan Lahan Di Kawasan Bandung Utara’, SoilREns, 15(1), pp. 38–45. https://doi.org/10.24198/soilrens.v15i1.13345.

Sonia, A.V. and Setiawati, T.C. (2022) ‘Aktivitas bakteri pelarut fosfat terhadap peningkatan ketersediaan fosfat pada tanah masam’, Agrovigor: Jurnal Agroekoteknologi, 15(1), https://doi.org/10.21107/agrovigor.v15i1.13449.

Taher, Y.A. (2021) ‘Dampak Pupuk Organik dan Anorganik terhadap Perubahan’, Jurnal Menara Ilmu, XV(2), pp. 67–76.

Triadiawarman, D., Aryanto, D. and Krisbiyantoro, J. (2022) ‘Peran Unsur Hara Makro Terhadap Pertumbuhan Dan Hasil Bawang Merah (Allium cepa L.)’, Agrifor, 21(1), p. https://doi.org/10.31293/agrifor.v21i1.5795.

Utami, S., Lidar, S. and Rizal, M. (2021) ‘Isolasi dan karakterisasi bakteri dan jamur pelarut fosfat pada berbagai lokasi’, Agrotela, 1(1), pp. 33–42.

Utarti, E. et al. (2021) ‘The Isolasi Aktinomiset Pelarut Fosfat Asal Perakaran Tembakau (Nicotiana tabacum L.) di Antirogo Jember’, Metamorfosa: Journal of Biological Sciences, 8(2), p. 260. https://doi.org/10.24843/metamorfosa.2021.v08.i02.p09.

Wahyumi, R., Hayati, R. and Agustine, L. (2023) ‘Uji Isolat Fungi Pelarut Fosfat dari Beberapa Jenis Tanah Terhadap Ketersediaan Hara Fosfat dan Pertumbuhan Tanaman Jagung pada Tanah Ultisol’, Jurnal Sains dan …, 5(2),. http://ejournal.sisfokomtek.org/index.php/saintek/article/view/1426%0Ahttp://ejournal.sisfokomtek.org/index.php/saintek/article/download/1426/1206.

Walida, H. et al. (2019) ‘Isolasi Dan Identifikasi Bakteri Penghasil Iaa Dan Pelarut Fosfat Dari Rhizosfer Tanaman Kelapa Sawit Isolation And Identification Of Iaa And Phosphate Solubilizing Bacteria From Palm Oil Rhizosphere PGPR ( Plant Growth Promoting PGPR beserta potensinya m’, 6(1), https://doi.org/10.31289/biolink.v6i1.2090.

Ye, L. et al. (2020) ‘Bio-organic fertilizer with reduced rates of chemical fertilization improves soil fertility and enhances tomato yield and quality’, Scientific Reports, 10(1), pp. 1–11. https://doi.org/10.1038/s41598-019-56954-2.

Yuan, H. et al. (2018) ‘Identification and expression profiling of the Aux/IAA gene family in Chinese hickory (Carya cathayensis Sarg.) during the grafting process’, Plant Physiology and Biochemistry, 127(December 2017), pp. https://doi.org/10.1016/j.plaphy.2018.03.010.